Generator

Part:BBa_K5319687:Design

Designed by: Zhang Liting   Group: iGEM24_ZJU-China   (2024-10-01)


VioC expression circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1965
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1965
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2633


Design Notes

Single RhlR may have a blocking effect on the downstream gene expression of specific promoter.


Source

VioC is derived from ATCC 21850.

References